Composite

Part:BBa_K887002:Design

Designed by: Chung, Chia-Jung   Group: iGEM12_NCTU_Formosa   (2012-09-21)

Plac+B0034+alsS+B0034+ilvC+B0034+ilvD+37℃RBS+tetR+double terminator+Ptet+B0032+kivD+B0015


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 6860
    Illegal XhoI site found at 5191
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 6080
    Illegal AgeI site found at 2560
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2146
    Illegal BsaI site found at 4977
    Illegal BsaI site found at 5234
    Illegal BsaI.rc site found at 534
    Illegal BsaI.rc site found at 1128
    Illegal BsaI.rc site found at 2965


Design Notes

The first part of this complete biobrick is built in Plac, two strong and one weak expressing RBS, Alss, ilvC, ilvD, 37°C regulator RBS, tetR and double terminator. The second part of this complete biobrick is start with Ptet,one RBS,and end with terminatorPart:BBa_B0015 is included.
We even try to improve this biobrick with encode zic finger genes before enzyme genes to make a fusion protein and make enzymes in order.When the intermediates are produced, it could have the next reaction as quick as possible.

Source

It is improved form NCTU-Formosa 2011 iGEM team.

References